A new integrated system combining atomic force microscopy and micro-electrode array for measuring the mechanical properties of living cardiac myocytes.
Identifieur interne : 000B06 ( Main/Exploration ); précédent : 000B05; suivant : 000B07A new integrated system combining atomic force microscopy and micro-electrode array for measuring the mechanical properties of living cardiac myocytes.
Auteurs : Jose F. Saenz Cogollo [Italie] ; Mariateresa Tedesco ; Sergio Martinoia ; Roberto RaiteriSource :
- Biomedical microdevices ; 2011.
English descriptors
- KwdEn :
- MESH :
- methods : Microscopy, Atomic Force.
- physiology : Cell Membrane, Elastic Modulus, Myocardial Contraction, Myocytes, Cardiac.
- ultrastructure : Myocytes, Cardiac.
- Animals, Microelectrodes, Rats, Stress, Mechanical.
Abstract
In this paper we present a new experimental set-up which combines the surface characterization capabilities of atomic force microscopy at the sub-micrometer scale with non-invasive electrophysiological measurements obtained by using planar micro-electrode arrays. In order to show the potential of the combined measurements we studied the changes in cell topography and elastic properties of cardiac muscle cells as during the contraction-relaxation cycle. The onset of each beating cycle was precisely identified by the use of the extracellular potential signal, allowing us to combine nanomechanical measurements from multiple cardiomyocyte contractions in order to analyze the time-dependent variation of cell morphology and elasticity. Moreover, by estimating the elastic modulus at different indentation depths in a single location on the cell membrane, we observed a dynamic mechanical behavior that could be related to the underlying myofibrillar structure dynamics.
DOI: 10.1007/s10544-011-9531-9
PubMed: 21455755
Affiliations:
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Saenz Cogollo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biophysical and Electronic Engineering, Università di Genova, Via Opera Pia 11a, 16145 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biophysical and Electronic Engineering, Università di Genova, Via Opera Pia 11a, 16145 Genova</wicri:regionArea><wicri:noRegion>16145 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Tedesco, Mariateresa" sort="Tedesco, Mariateresa" uniqKey="Tedesco M" first="Mariateresa" last="Tedesco">Mariateresa Tedesco</name></author><author><name sortKey="Martinoia, Sergio" sort="Martinoia, Sergio" uniqKey="Martinoia S" first="Sergio" last="Martinoia">Sergio Martinoia</name></author><author><name sortKey="Raiteri, Roberto" sort="Raiteri, Roberto" uniqKey="Raiteri R" first="Roberto" last="Raiteri">Roberto Raiteri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="doi">10.1007/s10544-011-9531-9</idno><idno type="RBID">pubmed:21455755</idno><idno type="pmid">21455755</idno><idno type="wicri:Area/PubMed/Corpus">000325</idno><idno type="wicri:Area/PubMed/Curation">000325</idno><idno type="wicri:Area/PubMed/Checkpoint">000328</idno><idno type="wicri:Area/Ncbi/Merge">000862</idno><idno type="wicri:Area/Ncbi/Curation">000862</idno><idno type="wicri:Area/Ncbi/Checkpoint">000862</idno><idno type="wicri:Area/Main/Merge">000B06</idno><idno type="wicri:Area/Main/Curation">000B06</idno><idno type="wicri:Area/Main/Exploration">000B06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new integrated system combining atomic force microscopy and micro-electrode array for measuring the mechanical properties of living cardiac myocytes.</title><author><name sortKey="Saenz Cogollo, Jose F" sort="Saenz Cogollo, Jose F" uniqKey="Saenz Cogollo J" first="Jose F" last="Saenz Cogollo">Jose F. Saenz Cogollo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biophysical and Electronic Engineering, Università di Genova, Via Opera Pia 11a, 16145 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biophysical and Electronic Engineering, Università di Genova, Via Opera Pia 11a, 16145 Genova</wicri:regionArea><wicri:noRegion>16145 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Tedesco, Mariateresa" sort="Tedesco, Mariateresa" uniqKey="Tedesco M" first="Mariateresa" last="Tedesco">Mariateresa Tedesco</name></author><author><name sortKey="Martinoia, Sergio" sort="Martinoia, Sergio" uniqKey="Martinoia S" first="Sergio" last="Martinoia">Sergio Martinoia</name></author><author><name sortKey="Raiteri, Roberto" sort="Raiteri, Roberto" uniqKey="Raiteri R" first="Roberto" last="Raiteri">Roberto Raiteri</name></author></analytic><series><title level="j">Biomedical microdevices</title><idno type="e-ISSN">1572-8781</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Membrane (physiology)</term><term>Elastic Modulus (physiology)</term><term>Microelectrodes</term><term>Microscopy, Atomic Force (methods)</term><term>Myocardial Contraction (physiology)</term><term>Myocytes, Cardiac (physiology)</term><term>Myocytes, Cardiac (ultrastructure)</term><term>Rats</term><term>Stress, Mechanical</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Atomic Force</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Membrane</term><term>Elastic Modulus</term><term>Myocardial Contraction</term><term>Myocytes, Cardiac</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Myocytes, Cardiac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Microelectrodes</term><term>Rats</term><term>Stress, Mechanical</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this paper we present a new experimental set-up which combines the surface characterization capabilities of atomic force microscopy at the sub-micrometer scale with non-invasive electrophysiological measurements obtained by using planar micro-electrode arrays. In order to show the potential of the combined measurements we studied the changes in cell topography and elastic properties of cardiac muscle cells as during the contraction-relaxation cycle. The onset of each beating cycle was precisely identified by the use of the extracellular potential signal, allowing us to combine nanomechanical measurements from multiple cardiomyocyte contractions in order to analyze the time-dependent variation of cell morphology and elasticity. 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